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mouse anti human cd58 mab  (R&D Systems)


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    Structured Review

    R&D Systems mouse anti human cd58 mab
    Mouse Anti Human Cd58 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+cd58+mab/Human+CD5+Antibody/pmc02675258-93-36-40
    Average 92 stars, based on 2 article reviews
    mouse anti human cd58 mab - by Bioz Stars, 2026-11
    92/100 stars

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    Incubation:

    Article Title: Human bronchial intraepithelial T cells produce interferon-? and stimulate epithelial cells
    Article Snippet: Samples were electrophoresed through 8% Tris-glycine gels (Invitrogen, Carlsbad, CA, USA) and transferred to nitrocellulose membranes (Amersham, Little Chalfont, Buckinghamshire, UK). .. Membranes were blocked with NET/gelatin (150 mM NaCl, 5 mM EDTA, 50 mM Tris, 0·05% Triton X-100, and 0·25% gelatin), and reacted with mouse anti-human E-cadherin mAb (BD Transduction Laboratories, San Diego, CA, USA) (1:1000) or mouse anti-human CD58 mAb (R&D systems) (1:1000) overnight at 4°C, then incubated with horseradish peroxidase-conjugated goat anti-mouse IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:5000) for 40 min at room temperature. .. The signals were developed using the Western Lightning Chemiluminescence Reagent (Perkin-Elmer Life Sciences, Boston, MA, USA) and exposed to Kodak BioMax films (Eastman Kodak, Rochester, NY, USA).



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